Lihlahisoa

Mycobacterium lefuba le ka tsoela pele ka lilemo tse mashome ho motho.Litsela tse matla tsa karabelo tse kenyelletsang inorganic polyphosphate [poly(P)], e kopantsoeng le ho etsoa ka hydrolyzed ke polyphosphate kinase (PPK) le exopolyphosphatase (PPX), ka ho latellana, ho lumeloa hore e bapala karolo ea bohlokoa ea taolo ho phehelleng baktheria.Re bontša mona hore M. tuberculosis poly(P) accumulation e amana ka nakoana le thibelo ea kholo ea bacillary.Re boetse re tsebahatsa M. lefuba Rv1026 e le novel exopolyphosphatase e nang le ts'ebetso ea hydrolytic khahlano le poly-chain (P) e telele.Re sebelisa tetracycline-inducible expression system ho theola polelo ea Rv1026 (ppx2), re fumane hore ho bokellana ha M. tuberculosis poly(P) ho lebisa ho holeng butle le ho fokotsa ts'oaetso ea isoniazid, ho hanyetsa mocheso le acid pH, le ho ntlafatsa bophelo ba intracellular. nakong ea tšoaetso ea macrophage.Ka microscope ea elektronike ea phetisetso, khatello ea ppx2 ea kokoana-hloko e bonts'itse botenya bo eketsehileng ba lerako la lisele, bo neng bo amahanngoa le ho fokotseha ho hoholo ha lerako la lisele ho lithethefatsi tsa hydrophilic ho fapana le ho kenya lipompo tsa efflux tsa lithethefatsi kapa sebopeho se fetotsoeng sa biofilm mabapi le taolo e se nang letho ea vector.Tlhahlobo ea transcriptomic le metabolomic e senotse ho fokotseha ha metabolism ea ppx2 knockdown e khetholloang ka ho fokotseha ha mongolo le phetolelo le ho fokotseha ha maemo a glycerol-3-phosphate.Ka kakaretso, poly(P) e phetha karolo ea bohlokoa thibelong ea kholo ea M. lefuba le metabolic downshift 'me e kenya letsoho ho mamellaneng ha lithibela-mafu ka ho fetoha ha lerako la lisele. ) ppGpp, ho lumeloa hore e sebetsa lipakeng tsa lefuba la Mycobacterium.Thutong ena, re khethile enzyme e ncha (Rv1026, PPX2) e ikarabellang bakeng sa hydrolyzing poly-chain poly(P).Lefuba la lefuba la M. le haelloang ke liphatsa tsa lefutso la ppx2 le bonts'itse ho eketseha ha maemo a poly(P), a neng a amahanngoa le ho ts'oaroa ha baktheria pele ho nako le ho fokotsa matla ho sethethefatsi sa pele sa isoniazid, hammoho le ho pholoha ha baktheria nakong ea khatello ea maikutlo. maemo le ka hare ho macrophages.Mabapi le khatello ea taolo, phetoho e bonts'itse botenya bo eketsehileng ba lerako la sele le ho fokotsa ho kenella ha lithethefatsi.Polelo ea liphatsa tsa lefutso lefatšeng ka bophara le tlhahlobo ea metabolite e senotse polelo e fokotsehileng ea mochini oa mongolo le oa phetolelo le phetoho tšebelisong ea mohloli oa khabone.Ka kakaretso, taolo ea tekanyo ea poly(P) ke ea bohlokoa bakeng sa ho tsoela pele ho thehoa ho M. lefuba.