Dithizone CAS: 60-10-6 80% phofo e putsoa e ntšo
| Nomoro ea lethathamo | XD90464 |
| Lebitso la Sehlahiswa | Dithizone |
| CAS | 60-10-6 |
| Foromo ea limolek'hule | C13H12N4S |
| Boima ba Molek'hule | 256.33 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29309098 |
Tlhaloso ea Sehlahisoa
| Tahlehelo ka ho Omisa | ≤5% |
| Ponahalo | Phofo e putsoa e ntšo |
| Tlhahlobo | 80% |
| Masalla a ho Ignition | ≤0.2% |
Mesenchymal stem cell (MSCs) e tlalehiloe e le mohloli o motle bakeng sa tlhahiso ea lisele tsa β tse ka fetisetsoang.Mohala oa sele ea embryonic mesenchymal progenitor cell C3H10T1/2 e amohetsoe e le mohlala oa li-MSC, ka lebaka la bokhoni ba eona ba ho arola mela e mengata.Sepheo sa phuputso ena e ne e le ho hlahloba hore na lisele tsa C3H/10T1/2 li na le monyetla oa ho khetholla lisele tse hlahisang insulin (IPCs).Mona, re ile ra batlisisa le ho bapisa phapang ea in vitro ea li-MSC tsa likhoto le lisele tsa C3H10T1/2 ho li-IPC.Kamora hore lisele li fumane karohano ea IPC, polelo ea matšoao a phapang e ile ea bonoa ke immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR (qRT-PCR) le Western blotting.Seteishene sa insulin se ile sa hlahlojoa ka enzyme-linked immunosorbent assay (ELISA).Ho feta moo, lisele tsena tse arohaneng li ile tsa fetisetsoa ho litoeba tse bakoang ke lefu la tsoekere la streptozotocin 'me mesebetsi ea tsona ea likokoana-hloko e ile ea lekoa ka har'a vivo.Phuputso ena e tlaleha mokhoa oa mekhahlelo e 2 oa ho hlahisa li-IPC ho tloha liseleng tsa C3H10T1/2.Tlas'a maemo a khethehileng a ho kenngoa ka matsatsi a 7-8, lisele tsa C3H10T1 / 2 li ile tsa theha 'mele oa spheroid oa mahlakore a mararo (SBs) le insulin e patiloeng, ha moloko oa IPC o tsoang ho rat MSCs o hloka nako e telele (ho feta libeke tse 2).Ho feta moo, li-IPC tsena tse nkiloeng ho lisele tsa C3H10T1/2 li ile tsa kenngoa ka har'a litoeba tsa lefu la tsoekere 'me tsa ntlafatsa basal glucose, boima ba' mele le ho bontša tlhahlobo e tloaelehileng ea ho mamella tsoekere.Boithuto ba hajoale bo fane ka mohlala o bonolo le o ts'epahalang oa in vitro bakeng sa ho etsa lipatlisiso ho ea pele mabapi le phapang ea IPC ea li-MSC le kalafo ea phetoho ea lisele bakeng sa lefu la tsoekere.
