Chymotrypsinogen A CAS:9035-75-0 phofo e tšoeu ea kristale
| Nomoro ea lethathamo | XD90376 |
| Lebitso la Sehlahiswa | Chymotrypsinogen A |
| CAS | 9035-75-0 |
| Foromo ea limolek'hule | C47H58FeN4O4S2 |
| Boima ba Molek'hule | 862.96 |
| Lintlha tsa polokelo | -20°C |
Tlhaloso ea Sehlahisoa
| Tlhahlobo | 99% |
| Ponahalo | Phofo e tšoeu ea kristale |
Adsorption ea liprotheine ho li-interfaces tse tiileng-mokelikeli o atisa ho fumanoa ka matla ho khothalletsa ho thehoa ha li-aggregates tse qhibilihang le likaroloana tse khōloanyane, tse bonahalang, le tse bonahalang, empa mehato ea bohlokoa ts'ebetsong ena hangata ho thata ho e hlahloba ka ho toba.Aggregation e kopantsoeng ke adsorption ho metsi-silicon oxide (SiOx) interfaces, ho tšoana le bokaholimo ba khalase ea hydrated, e ne e khetholloa e le mosebetsi oa pH le matla a ionic bakeng sa alpha-chymotrypsinogen (aCgn) le bakeng sa antibody ea monoclonal (IgG1).Sele e phallang e lumella neutron reflectivity bakeng sa likarolo tsa protheine tse khabisitsoeng ho hloekisa libaka tsa SiOx, hammoho le ka mor'a mehato e latellanang ea "rinse".Li-aggregates li ile tsa fumanoa ka tharollo ka mor'a "ho hlatsoa" ka bonolo holimo li ne li tšoauoa ka ho hasana ha neutron, microscopy le fluorescence spectroscopy.Limolek'hule tsa IgG1 li shebane haholo le "sephara" khahlano le bokaholimo ba SiOx, 'me karolo ea mantlha ea protheine e senyehile ho isa tekanyong e nyane, athe sekoaelo se pharalletseng se ne se hlatsuoa habonolo.Limolek'hule tsa aCgn li ne li hanana le ho senyeha ha li ne li bonahala eka li ka senoloa ka har'a sehokelo, empa li ne li tlosoa habonolo.Bakeng sa maemo ao ho nang le tlamahano e matla, protheine e entseng desorb e ne e le motsoako oa monoma le palo e nyane ea HMW aggregates (bakeng sa aCgn) kapa likaroloana tse ka tlase (bakeng sa IgG1).Liphetoho tsa adsorption le / kapa ho senoleha ka pH li bontšitse hore litšebelisano tsa motlakase li ne li le bohlokoa maemong 'ohle.
