Lihlahisoa

E sebelisoa ho etsa sekheo sa CAPS, buffer ea zwitterionic e sebetsang maemong a pH 7.9-11.1.CAPS buffer e sebelisoa haholo litekong tsa Bophirimela le tsa immunoblotting hammoho le tatellano ea protheine le boitsebahatso.E sebelisoa ho fetisetsa liprotheine tsa electro ho PVDF (sc-3723) kapa li-membrane tsa nitrocellulose (sc-3718, sc-3724).PH e phahameng ea buffer ena e etsa hore e be molemo bakeng sa phetiso ea liprotheine ka pI> 8.5.le reactivity e fokolang ka li-enzyme kapa liprotheine, liphello tse fokolang tsa letsoai.

Ho capillary zone electrophoresis lebelo la electrophoretic la ion le fokotseha ha mokelikeli oa motsoako oa mokokotlo oa electrolyte o ntse o eketseha.Sena se bakoa ke liphetoho tsa motsamao oa electrophoretic oa ion (muep) hammoho le liphetoho tsa matla a marang-rang a e amang, e leng matla a motlakase a sebetsang hantle (Eeff).Motsamao oa electrophoretic oa ion o fetoloa ka liphetoho tsa viscosity e felletseng ea tharollo ea electrolyte le liphetoho tsa boholo ba ion bo rarolitsoeng.Eeff e fetoloa haholo-holo ka liphetoho boholo ba tefiso ea asymmetry effect le phello ea electrophoretic, tseo ka bobeli li thibelang ho tsamaea ha li-ion.Thutong ena, mokhoa oa likarolo tse tharo o sebelisitsoe ho ithuta phello ea mokokotlo oa electrolyte (0.02-0.08M 3--[cyclohexylamino] -1-propanesulfonic acid le counter ion (Li, Na, K, le Cs) ho Eeff. Ho ile ha fumanwa hore kgatello ya bokamorao ba electrolyte bo ama Eeff haholo le hore Eeff e atamela E ha palo ya bokamorao ba electrolyte e atamela lefela. , Eeff e fokotsehile.