Lihlahisoa

Bicine ke zwitterionic amino acid buffer, e sebetsang ho pH 7.6-9.0 range (pKa ea 8.26 ho 25°C).Buffer e khothalelitsoeng bakeng sa mosebetsi o tlase oa mocheso oa biochemical.Bicine e sebelisoa bakeng sa ho lokisa tharollo e tsitsitseng ea substrate bakeng sa boikemisetso ba serum guanase.Tšebeliso ea bicine ka mokhoa o mosesaane oa ion exchange chromatography bakeng sa tharollo ea protheine e phatlalalitsoe.Bicine e sebelisitsoe ho peptide le protheine crystallization.Thuto ea kinetic ea quaternary transition-state analogue complex ea creatine kinase e sebelisitseng bicine ho buffer ea reaction.Sistimi ea li-multiphasic buffer bakeng sa SDS-PAGE ea liprotheine le li-peptide tse kenyelletsang bicine e hlalositsoe.

Mabota a le 'ngoe a alveolar a tlas'a lithuto tsa nako e telele ho saline le Bicine (0.2 M) a senyeha ho ea pele ho tsitsipano ea mesifa (TTD).Re hlahlobile phello ea litharollo tse fapaneng ho TTD ena mme ra batla liphetoho tse tsamaellanang ho ultrastructure.Lung parenchyma e ile ea aroloa ho mabota a alveolar a le mong (30 X 30 X 150 microns) ka phosphate-buffered saline (0.15 M).E fetiselitsoe ho bateng ea nako e telele, 'mele o ile oa qoelisoa ka har'a Bicine, saline, tharollo ea Hank e matlafalitsoeng, 0.25% Alcian blue ka saline, kapa sodium dodecyl sulfate solution, bakeng sa linako tse fapaneng.E tsamaellana le katoloso e fanoeng ka matla a phahameng a lekantsoeng ha nako e ntse e feta, li-tissue tsona tsena li ile tsa beoa ka har'a buffered glutaraldehyde/tannic acid mme tsa sebetsoa bakeng sa microscope ea elektrone.Mabota a le mong a alveolar a kenngoeng ka letsoai kapa Bicine a bontšitse TTD e tsoelang pele.Li-vacuoles kapa libaka li ile tsa hlaha ka har'a interstitium eo ka ho senyeha ha cellular ho ileng ha tsoela pele le TTD.E bonoa ka hare ho 0.3 h, liphetoho li ne li tsoetse pele ho 0.6 h.Ho sodium dodecyl sulfate (70 mM), leha ho le joalo, ho ne ho se na TTD mme ka sebopeho ho ne ho se na interstitium, ho setse feela lera le ka tlas'a lefatše le liprotheine tsa fibrous.Ka tharollo e matlafalitsoeng ea Hank kapa 0.25% Alcian blue, interstitial matrix, cell morphology le tsitsipano ea lisele li bolokiloe hantle bakeng sa 1 h.Phuputso ena e fana ka maikutlo a hore leaching ea matrix ea interstitial e etsahala ka letsoai kapa Bicine, 'me matrix e sa fetoheng e bohlokoa bakeng sa ho boloka tsitsipano ea lisele.