Monoclonal antibody (mAb 8281) e tobileng bakeng sa terminal β-galactose (βGal) ea glycosphingolipids (GSL) le glycoprotein e ile ea hlahisoa ho tloha litoeba tse kentsoeng ka lipid extract ho tloha liseleng tse ncha tsa acute lymphocytic leukemia (ALL).Immuno-thin layer chromatography (ITLC) le liteko tsa tlholisano tse nang le litekanyetso tse hloekisitsoeng tsa GSL tse sa jeleng paate, tsoekere e sa lefelloeng, le li-neoglycoprotein tsa maiketsetso li bontšitse mAb 8281 ho sebetsa ka matla ka LacCer, GalCer le Gal-β-O-(CH3)2S(CH3)2- CONH-(Gal-β-O-CETE) e hokahaneng le serum ea bovine albumin (BSA).Tsoekere e tsoetseng pele le eona e bile le karolo ho tlamang.Sesole sa 'mele se ne se sa sebetse ka lik'habohaedreite tse nang le li-terminal αGal le likarolo tse sa amaneng tsa terminal.Indirect immunoperoxidase staining and flow cytometry e nang le mAb 8281 e bonts'itse matheba a matle meleng e mengata, ho kenyeletsoa mesifa e boreleli, mala a mala, lymph node B cell le monocyte.Tlhahlobo ea ITLC ea sebopeho sa GSL sa li-neoplasms tse ncha tsa B tse sebelisang mAb 8281 li netefalitse boteng ba lactosylceramide le galactosylceramide ka li-neoplasms tsa mekhahlelo e fapaneng ea phapang.Ka lebaka la ho khetheha ha eona bakeng sa masala a "terminal βGal carbohydrate", mAb 8281 e ka ba molemo litlhahlobong tsa sebopeho le ts'ebetso ea GSL.