Li-isomeric bicyclo [4.1.0]heptane analogues tsa glycosidase inhibitor galacto-validamine, (1R*,2S,3S,4S,5S,6S*) -5-amino-1-(hydroxymethyl)bicyclo[4.1.0]heptane. -2,3,4-triol, e entsoe ka mehato ea 13 ho tloha 2,3,4,6-tetra-O-benzyl-D-galactose.Mesebetsi ea thibelo ea li-amine tse peli tse thibetsoeng ka mokhoa o lumellanang le tsona, le li-acetamide tse tsamaisanang le tsona, li ile tsa lekanngoa khahlanong le li-enzyme tsa khoebo tsa alpha-galactosidase ho tloha linaoa tsa kofi le E. coli.Mosebetsi oa glycosyl hydrolase lelapa la GH27 enzyme (linaoa tsa kofi) e ne e thibetsoe ka tlhōlisano ke 1R,6S-amine (7), tšebelisano e tlamang e neng e khetholloa ke boleng ba K (i) ba 0.541 microM.GH36 E. coli alpha-galactosidase e bontšitse tšebelisano e fokolang haholo e tlamang le 1R,6S-amine (IC(50)= 80 microM).Diastereomeric 1S,6R-amine (9) e hokahane ka bofokoli ho li-galactosidase ka bobeli, (naoa ea kofi, IC(50)= 286 microM) le (E. coli, IC(50)= 2.46 mM).