X-GAL CAS:7240-90-6 98% Powder ea Crystalline e tšoeu ho isa ho e tšoeu
| Nomoro ea lethathamo | XD90008 |
| Lebitso la Sehlahiswa | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Foromo ea limolek'hule | C14H15BrClNO6 |
| Boima ba Molek'hule | 408.63 |
| Lintlha tsa polokelo | -2 ho ea ho -6 °C |
| Khoutu ea Lekhetho e Kopantsoeng | 29400000 |
Tlhaloso ea Sehlahisoa
| Ponahalo ea Tharollo | Motsoako o hlakileng, o se nang 'mala ho isa ho o mosehla o khanyang (50mg/ml ho DMF:MeOH, 1:1) |
| Potoloho e khethehileng ea optical | -61.5 +/- 1 |
| Ponahalo | Phofo ea kristale e tšoeu ho isa bosoeu |
| Bohloeki HPLC | mets 99% |
| Sollubility (5 % ho DMF) | E qhibilihang (5% w/v,DMF) |
| Metsi KF | boholo 1% |
| Assay (HPLC on Anhydrous Basics) | mets 98% w/w |
Tšebeliso ea X-gal
X-gal (hape e khutsufalitsoe BCIG bakeng sa 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) ke motsoako oa tlhaho o nang le galactose e hokahaneng le indole e nkeloang sebaka.Motsoako ona o entsoe ke Jerome Horwitz le basebetsi-'moho ka 1964. Lebitso la k'hemik'hale le tloaelehileng hangata le khutsufatsoa ho isa ho tse sa nepahalang empa le lipoleloana tse fokolang tse kang bromochloroindoxyl galactoside.X e tsoang ho indoxyl e ka 'na ea e-ba mohloli oa X ho khohlano ea X-gal.X-gal e sebelisoa hangata ho baeloji ea limolek'hule ho etsa tlhahlobo ea boteng ba enzyme, β-galactosidase, sebakeng sa sepheo sa eona se tloaelehileng, e leng β-galactoside.E boetse e sebelisetsoa ho bona tšebetso ea enzyme ena ho histochemistry le bacteriology.X-gal ke e 'ngoe ea li-indoxyl glycosides le li-ester tse ngata tse hlahisang metsoako e putsoa e sa keneng e tšoanang le dae ea indigo ka lebaka la enzyme-catalyzed hydrolysis.
X-gal ke analoge ea lactose, ka hona e ka 'na ea e-ba le hydrolyzed ka enzyme ea β-galactosidase e khaolang tlamo ea β-glycosidic ho D-lactose.X-gal, ha e petsotsoe ke β-galactosidase, e hlahisa galactose le 5-bromo- 4-chloro-3-hydroxyindole - 1. E qetellang e fokotseha ka boithatelo 'me e kenngoa ka oxidized ho 5,5'-dibromo-4,4'-dichloro -indigo - 2, sehlahisoa se boputsoa haholo se sa qhibiliheng.X-gal ka boeona ha e na 'mala, kahoo boteng ba sehlahisoa sa 'mala o moputsoa bo ka sebelisoa e le teko ea boteng ba β-galactosidase e sebetsang.Sena se boetse se lumella baktheria β-galactosidase (eo ho thoeng ke lacZ ) ho sebelisoa e le motlalehi lits'ebetsong tse fapaneng.
Tlhahlobong ea li-hybrids tse peli, β-galactosidase e ka sebelisoa e le moqolotsi oa litaba ho tsebahatsa liprotheine tse sebelisanang.Ka mokhoa ona, lilaebrari tsa liphatsa tsa lefutso li ka hlahlojoa bakeng sa tšebelisano ea protheine ho sebelisoa tomoso kapa tsamaiso ea baktheria.Moo ho nang le tšebelisano e atlehileng lipakeng tsa liprotheine tse hlahlojoang, e tla fella ka ho tlamahanngoa ha sebaka sa ts'ebetso ho motšehetsi.Haeba motšehetsi a amana le lefutso la lacZ, tlhahiso ea β-galactosidase, e leng se etsang hore ho thehoe likolone tse nang le 'mala o moputsoa ka pel'a X-gal, kahoo li tla bontša tšebelisano e atlehileng pakeng tsa liprotheine.Mokhoa ona o ka lekanyetsoa feela ho hlahloba lilaebrari tsa boholo bo ka tlase ho 106. Ho phunyeha ho atlehileng ha X-gal ho boetse ho baka monko o monate ka lebaka la ho fetoha ha indole.
Kaha X-gal ka boeona ha e na 'mala, boteng ba sehlahisoa sa 'mala o moputsoa bo ka sebelisoa e le teko ea boteng ba β-galactosidase e sebetsang.
Khethollo ena e bonolo ea enzyme e sebetsang e lumella gene ea βgalactosidase (lacZ gene) hore e sebelisoe joalo ka moqolotsi oa litaba lits'ebetsong tse fapaneng.
