Tris maleate Cas: 72200-76-1 99.5% phofo e tšoeu ea kristale
| Nomoro ea lethathamo | XD90057 |
| Lebitso la Sehlahiswa | Tris maleate |
| CAS | 72200-76-1 |
| Foromo ea limolek'hule | C4H11NO3 · C4H4O4 |
| Boima ba Molek'hule | 237.21 |
| Lintlha tsa polokelo | Ambient |
| Khoutu ea Lekhetho e Kopantsoeng | 29221985 |
Tlhaloso ea Sehlahisoa
| Ponahalo | Phofo e tšoeu ea kristale |
| Tlhahlobo | ≥ 99.5% |
Cocrystallization ea lysyl-tRNA synthetase ho tloha Thermus thermophilus le tRNAlys ea eona e kopanetsoeng le Escherichia coli tRNAlys.
Lysyl-tRNA synthetase e tsoang ho Thermus thermophilus e kopantsoe le tRNAlys ea eona e kopaneng kapa Escherichia coli tRNAlys e sebelisang ammonium sulfate joalo ka precipitant.Likristale li hola ho tloha ho tharollo e nang le 1:2.5 stoichiometry ea synthetase dimer ho ea ho tRNA ka 18-22% ammonium sulfate ka 50 mM Tris-maleate buffer e pH 7.5.Li-complexes ka bobeli li etsa square prismatic, tetragonal crystals tse nang le li-unit cell parameters tse tšoanang haholo (a = b = 233 A, c = 119 A) 'me li fapane ho fihlela bonyane 2.7 A resolution.Leha ho le joalo homocomplex ke ea sehlopha sa sebaka P42(1)2 le heterocomplex ea sehlopha sa sebaka I422.
