Lihlahisoa

MicroRNA (miRNA) biosensor e nang le kutloelo-bohloko e sebelisang ruthenium oxide nanoparticle (RuO(2) NP) -e qalileng ho etsa polymerization ea 3,3'-dimethoxybenzidine (DB) le deposition e entsoeng ka miRNA ea insulating poly(3,3'-dimethoxybenzidine) (PDB) filimi e hlalosoa mosebetsing ona.Biosensor e entsoe ka monolayer e tsoakiloeng ea oligonucleotide capture probes (CPs) le 4-mercaptoaniline holim'a electrode ea khauta.Ka mor'a ho kopanya ka "MiRNA" e nang le RuO(2) NP-tagged target miRNA, motsoako oa DB/H(2)O(2) ka pH 5.0 0.10 M acetate buffer e ile ea sebelisoa ho biosensor.The RuO(2) NPs sebetsa e le polymerization initiator/catalyst bakeng sa polymerization ea DB.'Me likhoele tsa anionic miRNA tse nyalisitsoeng le li-CP tsa mahala li sebetsa e le litempele, tse tataisang ho beoa ha PDB.Palo ea PDB e kentsoeng le matla a eona a sireletsang li ne li amana ka kotloloho le mohopolo oa sepheo sa miRNA tharollong.Electrochemical impedance spectroscopic liteko li bontšitse hore linear charge-transfer resistance-concentration relationship from 6 .0 fM to 2.0 pM e ile ea finyelloa ka mor'a 60 min ea incubation motsoako oa DB/H(2)O(2).Ho ne ho se na cross-hybridization pakeng tsa pre-miRNA le miRNA e hōlileng ka ho feletseng le ho kopanya ho fokolang haholo har'a litho tse amanang haufi-ufi tsa lelapa la miRNA esita le maemong a sa lumellaneng a le mong.Biosensor ena e thehiloeng ho impedance e fana ka mokhoa o mong o khahlang bakeng sa profiling ea polelo ea miRNA mme e ka thusa ho nts'etsopele ea sistimi e nkehang ea li-multiplexing miRNA profiling.