Lihlahisoa

e hlalositse pejana hore molek'hule ea autoinducer, e tsejoang e le 1,3-diaminopropane (1,3-DAP), e hlahisoa ke Penicillium chrysogenum le Acremonium chrysogenum.Re sebelisa litloaelo tsa fermentor tse laoloang ke pH re bone mosebetsing ona hore 1,3-DAP le spermidine ka ho hlaka li susumetsa biosynthesis ea benzylpenicillin ho P. chrysogenum, ka bobeli ka mokhoa o hlalositsoeng le o rarahaneng oa tlhahiso ea penicillin.Ka bobeli 1,3-DAP le spermidine, empa eseng putrescine (1,4-diaminobutane), li hlahisa keketseho e kholo ea litekanyetso tsa transcript tsa penicillin biosynthetic gene pcbAB, pcbC le penDE.Li-polyamine tsena ha li ame polelo ea phalliso ea pH-stress regulator pacC ea lefats'e, kahoo e sa kenyeletse hore phello ea 1,3-DAP le spermidine e bakoa ke phetoho ea mokhoa oa ho laola pH.Polelo ea liphatsa tsa lefutso tse tharo tsa penicillin biosynthetic e fokotsehile haholo ka phetoho ea laeA-knock-down ea P. chrysogenum, e hlahisang maemo a tlase haholo a benzylpenicillin.Ho khahlisang, 1,3-DAP le spermidine li khutlisa phello ea phetoho ea laeA, e khutlisetsa ka botlalo maemo a tlhahiso ea penicillin.Ho feta moo, 1,3-DAP le spermidine li ntlafalitse polelo ea laeA khatellong ea botsoali le ho khutlisetsa maemo a mongolo oa laeA ka har'a phetoho ea laeA knock-down.Ha li bokelloa liphetho tsena li bonts'a hore phello e susumetsang ea limolek'hule tsa inducer 1,3-DAP le spermidine e sebelisoa, bonyane ka karolo e itseng, ka ho susumetsa polelo ea laeA, molaoli oa lefats'e o sebetsang ka mokhoa oa epigenetically polelong ea liphatsa tsa lefutso tsa bobeli tsa metabolite. ka ho hlophisoa bocha ha heterochromatin.