Lihlahisoa

Carbonic anhydrase activators: thuto ea pele ea ts'ebetso ea secretory ea motho ea isoform VI e nang le li-amino acid le li-amine.

The secretory isozyme of human carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, e entsoe cloned, e hlahisoa, 'me e hloekisitsoe tsamaisong ea baktheria.Mekhahlelo ea kinetic bakeng sa CO(2) hydration reaction e bontšitse hore hCA VI e na le ak(katse) ea 3.4 x 10(5)s(-1) le k(katse)/K(M) ea 4.9 x 10(7)M. (-1)s(-1) (ho pH 7.5 le 20 degrees C).hCA VI e na le ts'ebetso ea bohlokoa ea catalytic bakeng sa karabelo ea 'mele, ea tatellano ea boholo bo lekanang le isoform e fumanehang hohle CA I kapa transmembrane, isozyme e amanang le hlahala CA IX.Letoto la li-amino acid le li-amine li bonts'itsoe li sebetsa joalo ka li-activator tsa CA VI, tse nang le ts'ebetso e fapaneng.L-His, l-Trp, le dopamine li bontšitse liphello tse fokolang tsa CA VI tse sebetsang (K (A) s ka har'a 21-42 microM), athe d-His, d-Phe, l-DOPA, l-Trp, serotonin , 'me tse ling tsa pyridyl-alkylamine e ne e le li-activator tse molemo, tse nang le K (A) ka har'a 13-19 microM.Li-activator tse ntle ka ho fetisisa tsa CA VI e ne e le l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, le histamine, tse nang le K(A)s ho 1.23-9.31 microM.Lits'oants'o tsena kaofela li ntlafatsa k(katse), ha li na phello ho K(M), li nka karolo ka hona mohatong o khethollang oa potoloho ea catalytic, karabelo ea phetiso ea proton lipakeng tsa sebaka se sebetsang sa enzyme le tikoloho.