Lihlahisoa

Pokello ea nyutlelie ea mofuta o fetotsoeng oa protheine ea nyutlelie ea Lamin-A, e bitsoang Progerin (PG) kapa Lamin AΔ50, e etsahala Hutchinson-Gilford Progeria Syndrome (HGPS) kapa Progeria, lefu le tsofalang le potlakileng.E 'ngoe ea matšoao a ka sehloohong a lefu lena la lefutso ke tahlehelo ea mafura a ka tlas'a letlalo ka lebaka la lipodystrophy. phello ea polelo ea PG ka bokhoni ba phapang ea mohala ona oa sele, e 'ngoe ea mefuta e sebelisoang haholo ea adipogenic.Quantitative proteomics (iTRAQ) e entsoe ho ithuta phello ea ho bokella PG.Liprotheine tse 'maloa tse fetotsoeng li netefalitsoe ke immunoblotting le PCR ea nako ea nnete.Mosebetsi oa Mitochondrial o ile oa hlahlojoa ka tekanyo ea a) mosebetsi oa mitochondrial basal, b) tlhahiso ea anion ea superoxide le c) katleho ea motho ka mong ea mefuta e fapaneng ea ketane ea phefumoloho. ho fokotseha ha sekhahla sa ho ata le ho holofala ho matla a adipogenic ha ho bapisoa le taolo.Tlhahlobo ea proteomics e ngata e bonts'itse liprotheine tse 181 haholo (p <0.05) tse fetotsoeng ho PG-expressing preadipocytes.Ts'ebetso ea mitochondrial e holofetse liseleng tsa PG-expressing.Ka ho khetheha, re hlokometse keketseho ea ts'ebetso ea "I" e rarahaneng le tlhahiso e feteletseng ea superoxide anion.Incubation with Reactive Oxygen Species (ROS) scavenger agents (ROS) e lebisa phokotseho ea autophagic proteolysis joalokaha e senotsoe ke LC3-II/LC3-I ratio.PG expression in 3T3L1 cell e khothaletsa liphetoho mefuteng e mengata ea Biological process, ho kenyeletsoa sebopeho sa cytoskeleton, lipid metabolism, calcium. taolo, phetolelo, ho mena ha protheine le tlhahiso ea matla ke mitochondria.Lintlha tsa rona li matlafatsa monehelo oa ho bokella ROS ho phenotype ea botsofali pele ho nako le ho theha kamano pakeng tsa ho se sebetse ha mitochondrial le tahlehelo ea proteostasis ho HGPS.