Kanamycin A Sulfate CAS: 25389-94-0 phofo e tšoeu ea kristale
Nomoro ea lethathamo | XD90363 |
Lebitso la Sehlahiswa | Kanamycin A sulfate |
CAS | 25389-94-0 |
Foromo ea limolek'hule | C18H36N4O11 · H2O4S |
Boima ba Molek'hule | 582.58 |
Lintlha tsa polokelo | 15 ho isa ho 30 ° C |
Khoutu ea Lekhetho e Kopantsoeng | 29419000 |
Tlhaloso ea Sehlahisoa
Kereiti | USP |
pH | 6.5-8.5 |
Endotoxin ea baktheria | <0.60EU/mg |
Tahlehelo ka ho Omisa | 4.0% boholo |
Solubility | E qhibiliha ka bolokolohi metsing, e sa qhibilihe ka har'a acetone ho ethyl acetate le benzene |
Tlhahlobo | > 750ug/mg |
Molora oa Sulfate | 11 - 17.7% |
Masalla a ho Ignition | <1.0% |
Potoloho e khethehileng ea optical | +112 - +123 |
Metsoako e setseng | Ethanol 2000ppm Max |
Litšila ka Kakaretso | 3.0% boholo |
Ponahalo | Phofo e tšoeu ea kristale |
Letsatsi la Tlhahiso | TBC |
Kanamycin B | 1.5% boholo |
Tšilafalo efe kapa efe e sa tsejoeng | 0.45% boholo |
Moeli oa Likokoana-hloko | 25cfu/g Max |
Liprotheine / li-peptide tse thibelang likokoana-hloko li hohetse tlhokomelo e ngata ka lebaka la ts'ebeliso ea tsona e ka sebelisoang indastering.Thutong ea hajoale, protheine e thibelang likokoana-hloko e thermostable (BSAMP) e ile ea hloekisoa ho tloha setsong sa Bacillus subtilis FB123 ka ammonium sulfate precipitation, gel chromatography on Sephacryl S-200 High Resolution, le ion exchange chromatography ho DEAE Sepharose Fast Flow column.Boima ba molek'hule ea BSAMP e hloekisitsoeng e ne e le 54 kDa, joalo ka ha ho hakanngoa ke sodium dodecyl sulfate-polyacrylamide gel electrophoresis ka bobeli le boteng ba β-mercaptoethanol.Sebaka sa eona sa isoelectric se ne se ikemiselitse ho ba 5.24 ka isoelectric focusing electrophoresis.Periodic acid-Schiff staining e senotse BSAMP hore ke glycoprotein.Mosebetsi o ka holimo o fumanoe pH 6.0, 'me mosebetsi o fetang 79% o bolokiloe pH 3.0-5.0 le pH 7.0-9.0, ka ho latellana.BSAMP e bonts'itsoe e khona ho futhumatsa haholo, kaha ts'ebetso ea eona ha ea ka ea fetoha ka ho hlaka ka mor'a kalafo ka 100 ° C.Leha ho le joalo, e ne e sa amehe ka mokhoa o itseng ho papain, trypsin le alkali protease.Qetellong, protheine ea baktheria e ile ea bontša ts'ebetso ea antimicrobial e pharaletseng khahlanong le likokoana-hloko tse 'maloa.Liphuputso tsena li khothalelitse hore BSAMP e tsoele pele ho ntlafatsoa joalo ka sehlaseli sa tlhaho sa antibacteria bakeng sa thibelo ea mafu ho temo ea metsi le temo.