Lihlahisoa

E 'ngoe ea lintlha tse thibelang ts'ebeliso ea bongaka ea meriana ea ho tsosolosa ho mafu a fokolang a lefuba ke mohloli o loketseng oa lisele.Li-chondrocyte, mofuta o le mong oa lisele tsa lefufuru, tse hōlileng ka har'a vitro tlas'a maemo a setso ho atolosa lisele tsa lisele li lahleheloa ke phenotype ea tsona hammoho le bokhoni ba ho hlahisa lisele tsa hyaline cartilaginous.Thutong ena re etsa qeto ea hore mokhoa oa setso oa serum-le-factor-free-factor-free-dimensional three-dimensional (3D) o tsosolosa bokhoni ba li-chondrocyte tse fetisitsoeng ho etsa li-cartilage tissue in vitro, mokhoa o amanang le sox9.Bovine articular chondrocyte e ile ea fetisoa ka makhetlo a mabeli lumella ho atolosoa ha nomoro ea lisele (P2) le ho holisoa ka bongata bo phahameng ho li-membrane tse koahetsoeng ke 3D collagen-type-II-high glucose content media e tlatsitsoeng ka insulin le dexamethasone (SF3D).Lisele li ne li khetholloa ka mor'a ho atolosoa ha monolayer le ho latela setso sa 3D ka phallo ea cytometry, gene expression, le histology.Liphetoho tsa pele litseleng tsa ho fetisetsa lipontšo nakong ea redifferentiat ion li ne li khetholloa.P2 ho SF3D e hlahisitse liphatsa tsa lefutso tsa chondrogenic le matrix a extracellular.Ho theola insulin receptor (IR) ka HNMPA-(AM3) kapa tsela ea PI-3/AKT kinase (e sebelisoang ke kalafo ea insulin) ka Wortmannin e thibelang collagen synthesis.HNMPA-(AM3) e fokolitse polelo ea mofuta oa Col2, Col11, le IR hammoho le Sox6 le -9.Co-immunoprecipitation le chromatin immunoprecipitation analysis of HNMPA-(AM3) --treated cells li bonts'a tlamahano ea li-coactivators Sox6 le Med12 ka Sox9 empa e fokolitse ho tlama ha Sox9-Col2a1. Re hlalosa mokhoa o mocha oa setso o lumellang ho eketseha ha palo ea li-chondrocyte le ho khothalletsa sebopeho sa hyaline-like cartilage tissue ka karolo e itseng ka insulin-mediated Sox9-Col2a1 tlamang.Ho tšoaneleha ha lisele tse hlahisoang ka mokhoa ona bakeng sa tšebeliso ea ho lokisa manonyeletso ho hloka ho hlahlojoa ka vivo.