Lihlahisoa

Chemifluorescence: Luminol ea molek'hule ke molek'hule ea fluorescent ea lik'hemik'hale e ka fetoloang ho boemo bo thabisang ba aminophthalic acid ka pel'a limolek'hule tsa hydrogen peroxide, tse hlahisang fluorescence e matla.Hydrogen peroxide ke sehlahisoa sa lintho tse ngata tse amanang le biooxidative, kahoo ho bonolo ho hokahanya liketso tsena tsa biooxidative le photodetection ka ho hlahisa luminol.Mohlala, tlhahlobo ea glucose oxidase/catalase e ka lemoha bongata ba hydrogen peroxide kapa glucose sampole, mme nako ea karabo ke 0.5s feela (mokhoa o matla).Ho kopanya lintho tsa chemiluminescent le karabelo ea immunology, le ho bontša bongata ba likarolo tsa 'mele oa ho itšireletsa mafung ka karabelo e bobebe, ho bitsoa theknoloji ea chemiluminescent immunoassay.Ka 1976, Shmeder o ile a etsa tlhahiso ea chemiluminescence immunoassay, a sebelisa luminol-H202 le derivative ea eona ea ABEI e le mokhoa oa ho lemoha karabelo.Hajoale, luminol le li-derivatives tsa eona ke matšoao a sebelisoang haholo lits'ebetsong tsa chemiluminescence immunoassay.Tlas'a maemo a alkaline, a hlahisoang ke microperoxidase, palo e kholo ea photon e ka lokolloa.Luminol e ka sebelisoa bakeng sa WesternBlot ea li-antibodies tse ngotsoeng ke HRP le ho lemoha ha nucleic acid hybridization ea li-probe tse ngotsoeng ke HRP.E boetse e sebelisoa ho lemoha lefokisi la mali la sejoale-joale la botlokotsebe.Motsoako o ka sebelisoang e le letšoao la chemiluminescent o tlameha ho finyella maemo a latelang: tlhahiso ea quantum ea luminescence e phahame;thepa ea eona ea physicochemicals e lumellana le tsamaiso e ntseng e ithutoa;karabelo ea eona ea luminescent ke phello ea karabelo ea oxidation ea ntho e luminescent;ka har'a mefuta ea mahloriso e sebelisoang ka hare e se nang chefo ho lintho tse phelang.Mefuta e mengata ea li-reagents tsa chemiluminescence e atisa ho sebelisoa: acridine ester, chemiluminescence tracer e sebelisoang haholo, ke motsoako oa tricyclic, o bonolo ho oxidize, 'me oxidation reaction ha e hloke mokelikeli,' me e ntša lifoto ho 430nm;Luminol le isoluminol le lihlahisoa tsa tsona ke li-chemiluminescent tse hōlileng ka ho fetisisa.Hoo e ka bang ka 1964, Le hoja ho tlalehoa ka chemiluminescence, luminol, isoluminol le lihlahisoa tsa tsona li ne li sebelisetsoa chemiluminescence.Litlhahlobo tsa immunoassay li fumane liphello tse molemo.Ho lemoha ha luminescence: bolelele bo nepahetseng ba fluorescence wavelength ke 400nm (ho lemoha chemiluminescence ho 60mmK2S2O8, 100mmK2CO3, pH11.5 tharollo)

Chemiluminescence: Joalo ka mokhoa o hlokolosi oa tlhahlobo, chemiluminescence e sebelisoa haholo ho bona li-radicals tsa mahala le metabolites ea reaction e hlahisoang ka li-enzyme, lisele le likokoana-hloko.Leseli le hlahisoang ke lihlahisoa tsa bona tsa oxidation le metabolites li ka sebelisoa ka li-photometer tse fapaneng.ho lemoha.Chemiluminescence e sebelisoa haholo ho hlahloba le ho etsa lipatlisiso tsa li-antioxidants, tse kang polyphenols, polysaccharides, flavonoids, le anthraquinones, ka lebaka la kutloisiso ea eona, ho potlaka, ts'ebetso e bonolo le theko e tlaase.Litsamaiso tse sebelisoang hangata tsa chemiluminescence bakeng sa qeto ea li-anion tsa superoxide li kenyelletsa xanthine oxidase-luminol, pyrogallol-luminol le dimethyl sulfoxide-luminol.Ea pele ke tsamaiso ea enzymatic, 'me tse peli tsa morao ke tsamaiso e se nang enzymatic.Sistimi ea chemiluminescence bakeng sa qeto ea li-radicals tsa hydroxyl haholo-holo e kenyelletsa tomoso ea koporo ea sulfate (kapa lisele tsa moko oa masapo) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, koporo sulfate-o-phenanthroline-ascorbic acid-hydrogen. peroxide 5 tsamaiso ea chemiluminescence, ferrous sulfate-luminol-hydrogen peroxide le ferrous sulfate-luminol, kaofela li kenyelletsa mokhoa oa khale oa Fenton ho hlahisa li-radicals tsa hydroxyl, ebe o hlasela moemeli oa luminescent ho hlahisa chemiluminescence, e ka sebelisoang ho lekanya ho tlosoa ha li-extracts. ts'ebetso ea li-radicals tse sebetsang tsa oksijene.Mokhoa oa teko: sebelisa pyrogallol-luminol chemiluminescence system: luminol e entsoe ka tharollo ea 0.05mol / L ea mahloriso ka tharollo ea 0.05mol / L NaOH, e bolokiloeng sebakeng se lefifi, le metsi a mabeli a qhibilihisitsoeng pele a sebelisoa Dilute ho 1mmol / L tharollo, sebelisa 1mmol / L HCl ho etsa tharollo ea 0.01mol / L ea pyrogallol, e boloke ka sehatsetsing ka 4 ° C, 'me ue hlapolle ka metsi a mabeli a qhibilihisitsoeng ka makhetlo a 16 6.25 × 10-4mol / L tharollo pele o sebelisoa.0.05mol / L pH10.2Na2CO3-NaHCO3 buffer (e nang le 0.1mmol / LEDTA) e ile ea lokisoa pele e sebelisoa, 'me e kopantsoe le 1mmol / L luminol ho 2: 1 (karolo ea molumo) ho theha luminol le carbonate buffer pele ho motsoako oa liteko.Nakong ea tekanyo, kenya 10.0 μL ea lisampole tse fapaneng (0, 0.08, 0.4, 2 le 10 mg/mL) ka seleng ea luminescent (ka mohlala oa buffer e le taolo), ebe u kenya 6.25×10-4mol/L pyrogallol. 0.05 Qetellong, 0.94 mL ea motsoako oa luminol le carbonic acid buffer e ile ea kenngoa ho qala karabelo (30 ° C), matla a luminescence a ne a baloa ka nako ea 2 s, 'me kakaretso e kopantsoeng ea matla a luminescence ho 300 s e ile ea lekanngoa.Bokaholimo ba matla a luminescence e ne e le khanya ha pyrogallol e sa eketsoa.boleng.Ho phaella moo, ho na le chemiluminescence, fluorescence spectroscopy le NBS-dichlorofluorescein chemiluminescence tsamaiso bakeng sa qeto ea fluorescence chemiluminescence, joalo-joalo, molemo oa bona o moholo ke kutloisiso e phahameng.

Lintho tsa Lik'hemik'hale: Phofo ea kristale e mosehla.E qhibiliha ha bonolo ka har'a lye, e qhibiliha ka asiti e hlapollang, e batla e sa qhibilihe metsing, 'me ha e qhibilihe ke tai.Litharollo tse sa nke lehlakore kapa tse nang le asiti e nyane li bonts'a fluorescence e boputsoa bo khanyang ha e pepeselitsoe khanya ea ultraviolet.Sebaka se qhibilihang ke 329-332 ℃

Lisebelisoa: E le sesebelisoa sa tlhahlobo ea lik'hemik'hale le sesupo.Sebelisa reagent ea tlhahlobo ea Chemiluminescence (joalo ka qeto ea li-cations tsa tšepe kapa mali)

Lisebelisoa: Bakeng sa tlhahlobo ea chemiluminescence, joalo ka: cations ea tšepe, mali le glucocorticoids.

Lisebelisoa: Teko ea Luminescence: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; ka mor'a ho eketsa H2O2) chemiluminescence reagent le indicator, e sebelisoang hangata ho hlahloba chemiluminescence, joalo ka li-cations tsa tšepe, tšireletso ea mali, joalo-joalo.