Lihlahisoa

2,3,4,6-Tetrakis-O-trimethylsilyl-D-gluconolactone, eo ka tloaelo e tsejoang e le TMS-D-glucose, ke motsoako o nang le mefuta e mengata o fumanang likopo likarolong tse sa tšoaneng tsa saense, ho akarelletsa le motsoako oa lintho tse phelang, lik'hemik'hale tsa lik'habohaedreite le chemistry ea ho hlahloba. TMS-D-glucose e bohlokoa ka ho khetheha ho organic synthesis kaha e sebetsa e le sehlopha se sireletsang bakeng sa lihlopha tse sebetsang tsa hydroxyl (OH) ka lik'habohaedreite.Ka ho hlahisa lihlopha tsa trimethylsilyl (TMS) lihlopheng tsa hydroxyl tsa tsoekere, motsoako o fetoha o tsitsitseng le o fokolang, o lumellang phetoho e khethehileng ea lihlopha tse itseng tsa hydroxyl ha li ntse li siea ba bang ba sa amehe nakong ea liphetoho tsa lik'hemik'hale tse latelang.Leano lena la ts'ireletso le ts'ireletso le sebelisoa haholo k'hemik'hale ea lik'habohaedreite ho fihlela regioselectivity le stereochemistry ka har'a motsoako oa lik'habohaedreite tse rarahaneng, li-glycoconjugate le lihlahisoa tsa tlhaho. ea lik'habohaedreite.Ka ho fetola lik'habohaedreite hore e be lihlahisoa tsa tsona tsa trimethylsilyl, ho feto-fetoha ha tsona le botsitso ba mocheso li ntlafala, ho etsa hore li lokele ho hlahlojoa ka khase ea chromatography (GC) le mass spectrometry (MS).Mokhoa ona oa ho ntša metsi o ntlafatsa kutloisiso ea ho lemoha, o ntlafatsa katleho ea karohano, 'me o nolofalletsa ho tsebahatsa lik'habohaedreite tse fapa-fapaneng ka metsoako e rarahaneng, joalo ka lisampole tsa likokoana-hloko kapa lihlahisoa tsa lijo.TMS-D-glucose e boetse e fumana mekhoa ea ho kopanya li-reagents tse khethehileng le lipatlisiso tsa lik'hemik'hale.Reactivity ea eona e ikhethang le botsitso li etsa hore e be thepa ea bohlokoa ea ho qala bakeng sa ho lokisetsa metsoako e meng ea lik'habohaedreite.Bafuputsi ba ka fetola trimethylsilyl moiety kapa ba nkela sebaka sa glucose moiety ho etsa metsoako e nang le thepa e itseng, joalo ka li-fluorescent probes, enzyme inhibitors, kapa batho ba sebelisang lithethefatsi.Lintho tsena tse nkiloeng li ka sebelisoa lithutong tse fapa-fapaneng tsa likokoana-hloko le tsa likokoana-hloko, ho akarelletsa le litšoantšo, nts'etsopele ea lithethefatsi, kapa ho utloisisa ho sebelisana ha lik'habohaedreite le protheine. mehato ya tlhokomelo.Bafuputsi ba tlameha ho etsa bonnete ba hore ho na le moea o lekaneng le ho sebelisa lisebelisoa tse nepahetseng tsa ho itšireletsa ha ba sebetsa le motsoako ona ho thibela likotsi tse ka bang teng bophelong.Ho feta moo, joalo ka ha ho na le reagent efe kapa efe ea lik'hemik'hale, bohloeki le boleng ba TMS-D-glucose li bohlokoa ho fumana liphetho tse tšepahalang le tse ka hlahisoang hape.Bokhoni ba eona ba ho sireletsa lihlopha tsa hydroxyl ka lik'habohaedreite ka boikhethelo, ho sebetsa ha eona tlhahlobong ea lik'habohaedreite, le ts'ebeliso ea eona ho kopantseng li-reagents tse ikhethang li e etsa sesebelisoa sa bohlokoa lithutong tse fapaneng tsa mahlale.Ka ho sebelisa TMS-D-glucose, bafuputsi ba ka ntšetsa pele lithuto tsa bona ka k'hemistri ea lik'habohaedreite, glycoscience, le likarolo tse amanang le tsona, ho kenya letsoho ho nts'etsopele ea metsoako e mecha, tlhahlobo ea mali le lisebelisoa tsa kalafo.