Lihlahisoa

Ho ile ha etsoa aptasensor e sebetsang moo graphene oxide (GO) e ileng ea sebelisoa e le sesupo bakeng sa tlhahiso ea lipontšo tsa electrochemical impedance spectroscopy le electrochemiluminescence (ECL).Aptasensor e entsoe ka ho ipokellela probe ea ECL ea thiolated adenosine triphosphate binding aptamer (ABA) e tšoailoeng ka Ru complex (Ru(bpy)3(2+) derivatives) holim'a nanoparticle ea khauta (AuNP) e fetotsoeng khalase ea khabone. electrode (GCE).ABA e sa sisinyeheng ho AuNP e fetotsoeng GCE e ka bapatsa GO ka matla ka lebaka la tšebelisano e matla ea π-π lipakeng tsa ABA le graphene oxide;ECL quenching ea Ru complex e etsahala ka lebaka la ho fetisa matla le ho fetisoa ha elektronike, le keketseho e kholo ea ho hanyetsa ho fetisoa ha elektronike (Ret) ea electrode.Ha e ntse e le ka pel'a target adenosine triphosphate (ATP), ABA e khetha ho theha ABA-ATP bioaffinity complexes, e nang le kamano e fokolang le graphene oxide le ho boloka graphene oxide e le hole le holim'a electrode, kahoo e lumella ho ntlafatsa pontšo ea ECL, le mmoho le ho fokotseha ha Ret.Ka lebaka la ts'ebetso e phahameng ea ho tima ECL, sebopeho se ikhethang, le lisebelisoa tsa elektronike tsa graphene oxide, matla a Ret le ECL khahlanong le logarithm ea mahloriso a ATP a ne a lekana ka bophara ho tloha ho 10 pM ho ea ho 10 nM ka moeli oa ho lemoha o tlaase haholo oa 6.7 pM ho isa ho 4.8 pM, ka ho latellana.Aptasensor e reriloeng e bonts'itse ho ikatisa hantle, botsitso, le khetho e ikhethang, 'me ATP e ka khetholloa ka katleho ho li-analogue tsa eona.Habohlokoa le ho feta, leano lena le sebetsang hantle la ECL la aptasensor le ipapisitseng le GO e sebetsang joalo ka electrochemical le letšoao la lets'oao la ECL le akaretsang 'me le ka atolosetsoa habonolo liketsahalong tse ling tse tlamang baeloji.